P2871

Sigma

 

Monoclonal Anti-Cytokeratin, pan antibody produced in mouse

clone C-11, purified immunoglobulin, buffered aqueous solution

Synonym:Monoclonal Anti-Pan Cytokeratin

Description

Immunogenkeratin-enriched preparation from cultured human epidermoid carcinoma cell line A431.
SpecificityThe antibody recognizes human cytokeratins 4, 5, 6, 8, 10, 13, and 18. A broad spectrum antibody that reacts with a wide variety of normal, reactive, and neoplastic epithelial tissues. This antibody may be used for staining cultured epithelial cell lines.
Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody formpurified immunoglobulin
cloneC-11 (monoclonal)
formbuffered aqueous solution
species reactivitybovine, frog, human, rat, kangaroo rat, mouse
application(s)immunoblotting: suitable
 immunocytochemistry: 15-30 μg/mL using methanol/acetone-fixed PTK-2 cells
 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15-30 μg/mL using human skin
 immunohistochemistry (frozen sections): suitable
 microarray: suitable
isotypeIgG1
Gene Informationhuman ... KRT13(3858), KRT18(3875), KRT4(3851), KRT5(3852), KRT6A(3868), KRT6B(3854), KRT8(3856)
 rat ... Krt1-18(706059), Krt10(450225), Krt2-5(369017), Krt2-8(25626)
 mouse ... Krt10(16661), Krt13(16663), Krt18(16668), Krt4(16682), Krt5(110308), Krt6a(16687), Krt6b(16688), Krt8(16691)
shipped indry ice
storage temp.−20°C

Safety

WGK GermanyNWG

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References

referenceBartek, J., et al. J. Pathol. 164, 215, (1991)
 Bartkova, J., et al. Neoplasma 38, 439, (1991)
 Lane, E.B. and Alexander, C.M. Semin. Cancer Biol. 1, 165, (1990)